The analysis of whole blood preparations has obtained considerable popularity as a diagnostic and health monitoring tool in a wide range of alternative medicine fields. Dark ground and phase contrast techniques are commonly used. However blood cells are poor reflectors of light and have little by way of internal structure and therefore require very effective microscopy to obtain the the images that are needed. Standard biological microscopes are not sufficient for this application.
There are several units on the market that purport to offer the solution, and some are more effective than others. In essence the system requires two essential elements. Firstly an effective illumination system of at least 50 watts quartz halogen (LED illumination is not suitable) and secondly good quality optics. The correct balance between these two factors is essential to obtain the results needed to apply these techniques professionally and safely. It is not possible to purchase a suitable system cheaply, they just won’t do the job.
Illumination:
This is particularly important for dark ground analysis, which requires maximum illumination to overcome the fact that red blood cells are poor reflectors of light. A minimum of 50 watts is required for the light source, this is adequate providing the x100 objective is of good quality. Our own Brunel Haemascope has an excellent 100watt quartz halogen light source. Some other units attempt to overcome poorer quality objectives by introducing excessive light by using fibre optic illumination introduced into the condenser of the microscope. This usually causes flare (which produces images that are unreal) and also an uneven field illumination, and generally tries to mask the fact that the x100 objective of the system is just not of sufficient quality for the purpose.
Objectives:
The objectives of any microscope system are the essential components necessary to achieve the results required. Failure to address this fact by attempting to overcompensate with unnecessary over illumination is a significant criticism of some units. For dark ground analysis, both units which Brunel Microscopes recommend incorporate high quality plan x100 objectives with an internal iris diaphragm. For phase contrast examination only the best will do. Both systems offered have excellent phase contrast systems that deliver the results required.
Other Factors:
Attention to detail is all important, and the types of slides, coverslips and even immersion oil can have a significant effect. Brunel Microscopes supply, with both its recommended systems, the best quality slides and coverslips of the correct thickness and our own formulated immersion oil.
Both systems offered by Brunel Microscopes address all these issues and provide the optical results required at a very competitive price.